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KMID : 0644120110240020033
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Journal of Oriental Gynecology
2011 Volume.24 No. 2 p.33 ~ p.51
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Protective Effect of Processed Saengmaek-san(SM) on Cell Damage in UV-exposed HaCaT Cell
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Kim Eun-Seop
Yoo Dong-Youl
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Abstract
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Objectives: This study was performed to assess the protective effect of Saengmaek-san (SM) on UVB-induced HaCaT cell damage.
Methods:The protective effects of Saengmaek-san(SM) were determined by UVB-induced HaCaT assay. We assessed protective effects of Saengmaek-san (SM) on LDH release and nitrite release from HaCaT. And COX-2, Bcl-2, Bax, TNFα, c-jun, c-fos, NF-kB, iNOS, Bcl-xL gene expression were determined in HaCaT using real-time PCR method.
Results: 1. SM inhibited LDH-release, nitrite production in UVB-exposed HaCaT. 2. SM suppressed the gene expression of COX-2, TNFα in UVB-exposed HaCaT. 3. SM increased the gene expression of Bcl-2, Bax, Bcl-xL family protein in UVBexposed HaCaT. 4. SM suppressed the gene expression of c-jun, c-fos, NF-kB in UVB-exposed HaCaT.
Conclusions :The study showed SM inhibited the cell damage in UVB-exposed HaCaT.
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KEYWORD
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Saengmaek-san(SM), Protective effect, Gene expression
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